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Features & Capabilities

 

The Need for New Liposome Development Tools in the Research Community:


The liposome research community needs new production tools so that novel designed liposomes of therapeutic use can make it to production. Low pressure vortex mixing and low pressure extrusion present an alternative to high pressure membrane extrusion. The Hashimoto Liposome Automaker expands the possibilities for liposome process development, production, and scale-up by incorporating orthodox methods within a fully automated configurable system. The release of the Hashimoto Liposome Automaker enables a new generation of liposome breakthroughs for the research community.

 

Unique Features and Capabilities of Automated Multi-functional Liposome Manufacturing Equipment

 

1. Whole parts

 

1A. Preparation

 

(1) The Hashimoto Liposome Automaker uses orthodox methods of low pressure vortex mixing to make highly reproducible liposomes of all varieties, putting tremendous power in the hands of the end user. Even unskilled users can prepare liposomes only with the touch of button. Scientists and technicians don't need to become liposome expert to make reproducible stable liposomes. The Hashimoto Liposome Automaker's proprietary processes manage critical settings and conditions to empower the liposome researcher.

 

(2) Whole procedures such as patented lipid-film formation and liposome preparation are operated by vortex-mixing only. The addition of ultrasonication allows for SU(M)V preparation.

 

(3) Unilamellar vesicles can be prepared easily under the conditions of low lipid concentration and low salt concentration.

 

(4) Liposomes can be prepared under oxygen-free and germ-free conditions.

 

(5) The Hashimoto Liposome Automaker can prepare liposome in only one hour, whereas manual methods would take up to ten hours.

 

(6) Preparation is fully reproducible. Identical levels of liposome preparations can be achieved and repeated up to ten times.

 

(7) Temperature of sample tube can be fixed above phase-transition temperature, maximum 60 centigrade, so that DSPC can be easily treated.

 

(8) Solvent removal by vortex-mixing can avoid undesirable bumps and irregularities, which often occur with the use of rotary evaporator.

 

(9) The Hashimoto Liposome Automaker functions as a bioreactor, allowing for example, enzyme-substrate reactions and conjugation for mass production of bio-active substances.

 

(10)The operational conditions can be arbitrarily set, selected, and recorded. Conditions can be varied and controlled in order to achieve optimal conditions and results, providing a unique and efficient environment for liposome process development. The reproducible environment is ideal for proof-of-concept and pre-clinical process optimization to save time and reduce development costs. The Hashimoto Liposome Automaker can be qualified for cGMP environments.

 

1B. Incorporation

 

(1) The Hashimoto Liposome Automaker can prepare liposomes at higher lipid concentrations, enabling
higher trapping efficiencies for small molecules, antibodies, and nucleic acids.

 

(2) Oil-soluble substances such as curcumin and protoporphyrin IX can be completely incorporated into liposome membranes.

 

(3) Substance-incorporated liposomes are stable (non-leaky) for at least three or four months.

 

1C. Binding

 

(1) More than 90% of proteins and peptides can be bound covalently to liposome membranes with high
binding ratio.

 

(2) Protein- or peptide-bound, substance-encapsulated liposomes are also stable (non-leaky) for at least

three or four months.

 

2. Individual parts

 

2A. MLV

 

(1) MLV can be prepared easily and reproducibly using only conventional vortex-mixing.

 

(2) The Hashimoto Lipsome Automaker allows for variable control of vortex mixing.

Larger and smaller sizes of liposomes can be obtained by lower and higher revolution and by shorter and longer mixing intervals.

 

2B. SU(M)V

 

(1) Less than 70nm-sized liposomes, which are stable and clear for at least for 6 months, can be obtained using conventional ultrasonication.

 

(2) Temperature control of homogenizer can be set between 5 and 60 centigrade.

 

2C. LU(M)V

 

(1) LU(M)V can be prepared using modified reverse-phase evaporation method: W/O emulsion can be obtained by vortex-mixing instead of bath-type ultrasonication.

 

(2) Solvent removal by vortex-mixing under reduced pressure can avoid gelation, which results in phase-transitional bilayer formation.

 

(3) The size of the resulting LU(M)V is around 130 nm, which is suitable for cancer therapy.

 

2D. GU(M)V

 

(1) GU(M)Vs are prepared using a novel sugar-doped lipid-film hydration method, which can produce large amounts of stable and average 20┬Ám-sized liposomes.

 

(2) On incorporation of glycolipid, the size of GU(M)V could be higher.

 

(3) The Hashimoto Liposome Automaker allows critical control of phospholipid ratios. At an increasing
ratio of acidic phospholipids to total phospholipids, the amount of GUV can be increased.

 

(4) Helical or rod structure of GU(M)V, which is often observed during gentle hydration, can be

avoided at high ratios of sugar to lipid.

 

 

Video
AutoMaker Video

 

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Manual vs. AutoMaker Liposome Making Slideshow